confocal fluorescence microscope (Olympus)
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Olympus
confocal fluorescence microscope
Confocal Fluorescence Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 24380 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+fluorescence+microscope/FV3000+Confocal+Laser+Scanning+Microscope/pmc12996665-310-28-32
Average 99 stars, based on 24380 article reviews
Confocal Fluorescence Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 24380 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+fluorescence+microscope/FV3000+Confocal+Laser+Scanning+Microscope/pmc12996665-310-28-32
Average 99 stars, based on 24380 article reviews
confocal fluorescence microscope - by Bioz Stars,
2026-09
99/100 stars
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Staining:Article Title: Dual antagonism of a novel Streptomyces species against banana anthracnose by ROS-mediated direct inhibition and host priming Article Snippet: Postharvest banana disease caused by Colletotrichum musae leads to significant economic and nutritional losses.. While antagonistic microorganisms offer a promising control strategy, their practical efficacy is often constrained.. Discovering novel antagonistic species and clarifying their interaction mechanism remain essential. Article Title: Evaluation of the efficiency of double recombinant vaccinia virus VV-GMCSF-Lact against glioblastoma using 3D spheroid and cerebral organoid models Article Snippet: Then, 100 μL of secondary antibodies, including Donkey Anti-Rabbit IgG H&L (ab150075, Abcam, United Kingdom), Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A32727, Thermo Fisher Scientific, USA), for 2 h at RT on a shaker at 150 rpm. .. Afterward, the buffer with secondary antibodies was removed, and the wells were washed three times with 200 μL of cold PBS, each wash lasting 20 min. Stained and air-dried sections were treated with antifade solution, covered with coverslips, and visualized using a Article Title: Thrombin-mediated increased permeability of blood-brain barrier in diquat poisoning: Mechanistic insights and potential therapeutic targets. Article Snippet: For the colocalization of Ca2+ with the ER, Ca2+ and ER were labeled with the Ca2+ green fluorescent probe (Solarbio Life Sciences, Beijing, China) and ER red fluorescent probe (Beyotime Biotechnology, Shanghai, China) in HCMEC/D3 cells. .. After washing with HBSS, cells were incubated with Hoechst 33342 (Solarbio Life Sciences, Beijing, China) to stain the nuclei and observed using a Fluorescence:Article Title: Dual antagonism of a novel Streptomyces species against banana anthracnose by ROS-mediated direct inhibition and host priming Article Snippet: Postharvest banana disease caused by Colletotrichum musae leads to significant economic and nutritional losses.. While antagonistic microorganisms offer a promising control strategy, their practical efficacy is often constrained.. Discovering novel antagonistic species and clarifying their interaction mechanism remain essential. Article Title: Evaluation of the efficiency of double recombinant vaccinia virus VV-GMCSF-Lact against glioblastoma using 3D spheroid and cerebral organoid models Article Snippet: Then, 100 μL of secondary antibodies, including Donkey Anti-Rabbit IgG H&L (ab150075, Abcam, United Kingdom), Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A32727, Thermo Fisher Scientific, USA), for 2 h at RT on a shaker at 150 rpm. .. Afterward, the buffer with secondary antibodies was removed, and the wells were washed three times with 200 μL of cold PBS, each wash lasting 20 min. Stained and air-dried sections were treated with antifade solution, covered with coverslips, and visualized using a Article Title: Oligomer-dependent and -independent pathogenesis of muscular dystrophy-associated mutations within the penta-EF-hand domain of calpain-3. Article Snippet: After washing with PBS for 15 min in total, the cells were probed with goat antirabbit IgG (H+L) cross-adsorbed secondary antibody Alexa FluorTM 488 (Thermo Fisher, A-11008, 1:1000) and goat anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody Alexa FluorTM 594 (Thermo Fisher, A-11032, 1:1000) for 30 min at RT. .. After three additional PBS washes (15 min in total), the cells were mounted with Vibrance Antifade Mounting Medium containing 4’,6-diamidino-2-phenylindole (DAPI; Vector Laboratories, Inc., CA, USA, H-1800) and imaged using a Article Title: Human umbilical cord mesenchymal stem cells alleviate hypoxic-ischemia-induced white-matter injury in neonatal rats by regulating polarization of microglia. Article Snippet: The primary antibodies used were Iba1 antibody (1:100, ab283319; Abcam), CD86 antibody (1:100, BM4121; Boster, Wuhan, China), CD206 antibody (1:200, ab64693; Abcam), iNOS antibody (1:200, 80517-1-RR; Proteintech, IL, USA), NLRP3 (1:100, WL02635; Wanleibo, China), TLR4 (1:100, WL00196; Wanleibo, China) and Arg-1 antibody (1:100, A01106; Boster, Wuhan, China). .. Fluorescence images were captured using a Article Title: Jiangcaoxiang alleviates hepatic fibrosis through the Nrf2/GPX4 signaling pathway: Elucidated via integrated network pharmacology, multi-omics profiling, and experimental validation. Article Snippet: .. Following washes with PBS, the secondary antibody was introduced for a 1-h incubation period, which was then followed by observation and imaging under a Article Title: Leveraging self-healing CASH gel for enhanced paracrine secretion of mesenchymal stem cells to boost endometrial regeneration. Article Snippet: Locally delivering mesenchymal stem cells (MSCs) has been recently demonstrated to be successful in the treatment of intrauterine adhesions (IUA) by taking advantage of the immunomodulatory, pro-angiogenic, and anti-fibrotic properties of MSCs to facilitate endometrial regeneration and restore uterine function.. However, direct exposure of these donor cells to the detrimental pathological microenvironment in the uterine cavity invariably leads to a low cell survival rate, poor control over cell fates, and impaired cellular functionalities, which can significantly compromise the therapeutic efficacy.. Herein, we proposed a gelatin-based, colloidassembled self-healing (CASH) hydrogel to deliver and retain the donor MSCs locally in the uterine cavity. Article Title: Oligomer-dependent and oligomer-independent pathogenesis of muscular dystrophy-associated mutations within the penta-EF-hand domain of calpain-3 Article Snippet: After washing with PBS for 15 min in total, the cells were probed with goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody Alexa Fluor 488 (Thermo Fisher, A-11008, 1:1000) and goat anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody Alexa Fluor 594 (Thermo Fisher Scientific, A-11032, 1:1000) for 30 min at RT. .. After three additional PBS washes (15 min in total), the cells were mounted with Vibrance Antifade Mounting Medium containing 4′,6-diamidino-2-phenylindole (Vector Laboratories, Inc, H-1800) and imaged using a Article Title: Thrombin-mediated increased permeability of blood-brain barrier in diquat poisoning: Mechanistic insights and potential therapeutic targets. Article Snippet: For the colocalization of Ca2+ with the ER, Ca2+ and ER were labeled with the Ca2+ green fluorescent probe (Solarbio Life Sciences, Beijing, China) and ER red fluorescent probe (Beyotime Biotechnology, Shanghai, China) in HCMEC/D3 cells. .. After washing with HBSS, cells were incubated with Hoechst 33342 (Solarbio Life Sciences, Beijing, China) to stain the nuclei and observed using a Microscopy:Article Title: Dual antagonism of a novel Streptomyces species against banana anthracnose by ROS-mediated direct inhibition and host priming Article Snippet: Postharvest banana disease caused by Colletotrichum musae leads to significant economic and nutritional losses.. While antagonistic microorganisms offer a promising control strategy, their practical efficacy is often constrained.. Discovering novel antagonistic species and clarifying their interaction mechanism remain essential. Article Title: Evaluation of the efficiency of double recombinant vaccinia virus VV-GMCSF-Lact against glioblastoma using 3D spheroid and cerebral organoid models Article Snippet: Then, 100 μL of secondary antibodies, including Donkey Anti-Rabbit IgG H&L (ab150075, Abcam, United Kingdom), Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A32727, Thermo Fisher Scientific, USA), for 2 h at RT on a shaker at 150 rpm. .. Afterward, the buffer with secondary antibodies was removed, and the wells were washed three times with 200 μL of cold PBS, each wash lasting 20 min. Stained and air-dried sections were treated with antifade solution, covered with coverslips, and visualized using a Article Title: Oligomer-dependent and -independent pathogenesis of muscular dystrophy-associated mutations within the penta-EF-hand domain of calpain-3. Article Snippet: After washing with PBS for 15 min in total, the cells were probed with goat antirabbit IgG (H+L) cross-adsorbed secondary antibody Alexa FluorTM 488 (Thermo Fisher, A-11008, 1:1000) and goat anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody Alexa FluorTM 594 (Thermo Fisher, A-11032, 1:1000) for 30 min at RT. .. After three additional PBS washes (15 min in total), the cells were mounted with Vibrance Antifade Mounting Medium containing 4’,6-diamidino-2-phenylindole (DAPI; Vector Laboratories, Inc., CA, USA, H-1800) and imaged using a Article Title: Human umbilical cord mesenchymal stem cells alleviate hypoxic-ischemia-induced white-matter injury in neonatal rats by regulating polarization of microglia. Article Snippet: The primary antibodies used were Iba1 antibody (1:100, ab283319; Abcam), CD86 antibody (1:100, BM4121; Boster, Wuhan, China), CD206 antibody (1:200, ab64693; Abcam), iNOS antibody (1:200, 80517-1-RR; Proteintech, IL, USA), NLRP3 (1:100, WL02635; Wanleibo, China), TLR4 (1:100, WL00196; Wanleibo, China) and Arg-1 antibody (1:100, A01106; Boster, Wuhan, China). .. Fluorescence images were captured using a Article Title: Jiangcaoxiang alleviates hepatic fibrosis through the Nrf2/GPX4 signaling pathway: Elucidated via integrated network pharmacology, multi-omics profiling, and experimental validation. Article Snippet: .. Following washes with PBS, the secondary antibody was introduced for a 1-h incubation period, which was then followed by observation and imaging under a Article Title: Leveraging self-healing CASH gel for enhanced paracrine secretion of mesenchymal stem cells to boost endometrial regeneration. Article Snippet: Locally delivering mesenchymal stem cells (MSCs) has been recently demonstrated to be successful in the treatment of intrauterine adhesions (IUA) by taking advantage of the immunomodulatory, pro-angiogenic, and anti-fibrotic properties of MSCs to facilitate endometrial regeneration and restore uterine function.. However, direct exposure of these donor cells to the detrimental pathological microenvironment in the uterine cavity invariably leads to a low cell survival rate, poor control over cell fates, and impaired cellular functionalities, which can significantly compromise the therapeutic efficacy.. Herein, we proposed a gelatin-based, colloidassembled self-healing (CASH) hydrogel to deliver and retain the donor MSCs locally in the uterine cavity. Article Title: Oligomer-dependent and oligomer-independent pathogenesis of muscular dystrophy-associated mutations within the penta-EF-hand domain of calpain-3 Article Snippet: After washing with PBS for 15 min in total, the cells were probed with goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody Alexa Fluor 488 (Thermo Fisher, A-11008, 1:1000) and goat anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody Alexa Fluor 594 (Thermo Fisher Scientific, A-11032, 1:1000) for 30 min at RT. .. After three additional PBS washes (15 min in total), the cells were mounted with Vibrance Antifade Mounting Medium containing 4′,6-diamidino-2-phenylindole (Vector Laboratories, Inc, H-1800) and imaged using a Article Title: Thrombin-mediated increased permeability of blood-brain barrier in diquat poisoning: Mechanistic insights and potential therapeutic targets. Article Snippet: For the colocalization of Ca2+ with the ER, Ca2+ and ER were labeled with the Ca2+ green fluorescent probe (Solarbio Life Sciences, Beijing, China) and ER red fluorescent probe (Beyotime Biotechnology, Shanghai, China) in HCMEC/D3 cells. .. After washing with HBSS, cells were incubated with Hoechst 33342 (Solarbio Life Sciences, Beijing, China) to stain the nuclei and observed using a Incubation:Article Title: Jiangcaoxiang alleviates hepatic fibrosis through the Nrf2/GPX4 signaling pathway: Elucidated via integrated network pharmacology, multi-omics profiling, and experimental validation. Article Snippet: .. Following washes with PBS, the secondary antibody was introduced for a 1-h incubation period, which was then followed by observation and imaging under a Article Title: Thrombin-mediated increased permeability of blood-brain barrier in diquat poisoning: Mechanistic insights and potential therapeutic targets. Article Snippet: For the colocalization of Ca2+ with the ER, Ca2+ and ER were labeled with the Ca2+ green fluorescent probe (Solarbio Life Sciences, Beijing, China) and ER red fluorescent probe (Beyotime Biotechnology, Shanghai, China) in HCMEC/D3 cells. .. After washing with HBSS, cells were incubated with Hoechst 33342 (Solarbio Life Sciences, Beijing, China) to stain the nuclei and observed using a Imaging:Article Title: Jiangcaoxiang alleviates hepatic fibrosis through the Nrf2/GPX4 signaling pathway: Elucidated via integrated network pharmacology, multi-omics profiling, and experimental validation. Article Snippet: .. Following washes with PBS, the secondary antibody was introduced for a 1-h incubation period, which was then followed by observation and imaging under a |